Components of the yeast spindle and spindle pole body.
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چکیده
منابع مشابه
Components of the yeast spindle and spindle pole body
Yeast spindle pole bodies (SPBs) with attached nuclear microtubles were enriched approximately 600-fold from yeast cell extracts. 14 mAbs prepared against this enriched SPB fraction define at least three components of the SPB and spindle. Immunofluorescent staining of yeast cells showed that throughout the cell cycle two of the components (110 and 90 kD) were localized exclusively to the SPB re...
متن کاملSpindle pole body components are reorganized during fission yeast meiosis
During meiosis, the centrosome/spindle pole body (SPB) must be regulated in a manner distinct from that of mitosis to achieve a specialized cell division that will produce gametes. In this paper, we demonstrate that several SPB components are localized to SPBs in a meiosis-specific manner in the fission yeast Schizosaccharomyces pombe. SPB components, such as Cut12, Pcp1, and Spo15, which stay ...
متن کاملDirect interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body
The Saccharomyces cerevisiae genes KAR1 and CDC31 are required for the initial stages of spindle pole body (SPB) duplication in yeast. The Cdc31 protein is most related to caltractin/centrin, a calcium-binding protein present in microtubule organizing centers in many organisms. Because of a variety of genetic interactions between CDC31 and KAR1 (Vallen, E. A., W. Ho. M. Winey, and M. D. Rose. 1...
متن کاملDirect Interaction between Yeast Spindle Pole Body Components: Karlp Is Required for Cdc31p Localization to the Spindle Pole Body
The Saccharomyces cerevisiae genes KAR/ and CDC31 are required for the initial stages of spindie pole body (SPB) duplication in yeast. The Cdc31 protein is most related to caltractin/centrin, a calciumbinding protein present in microtubule organizing centers in many organisms. Because of a variety of genetic interactions between CDC31 and KARl (VaUen, E. A., W. Ho, M. Winey, and M. D. Rose. 199...
متن کاملLessons from yeast: the spindle pole body and the centrosome.
The yeast spindle pole body (SPB) is the functional equivalent of the centrosome. Most SPB components have been identified and their functions partly established. This involved a large variety of techniques which are described here, and the potential use of some of these in the centrosome field is highlighted. In particular, very useful structural information on the SPB was obtained from a reco...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1990
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.111.5.1913